Spatially restricted substrate-binding site of cortisol-synthesizing CYP11B1 limits multiple hydroxylations and hinders aldosterone synthesis
CURRENT RESEARCH IN STRUCTURAL BIOLOGY(2021)
摘要
Human cytochromes P45011 beta (CYP11B1) and P450(aldo) (CYP11B2) are monooxygenases that synthesize cortisol through steroid 11 beta-hydroxylation and aldosterone through a three-step process comprising 11 beta-hydroxylation and two 18-hydroxylations, respectively. CYP11B1 also catalyzes 18-monohydroxylation and 11 beta,18-dihydrox-ylation. To study the molecular basis of such catalytic divergence of the two enzymes, we examined a CYP11B1 mutant (Mt-CYP11B1) with amino acid replacements on the distal surface by determining the catalytic activities and crystal structure in the metyrapone-bound form at 1.4-angstrom resolution. Mt-CY11B1 retained both 11 beta-hydrox-ylase and 18-hydroxylase activities of the wild type (Wt-CYP11B1) but lacked 11 beta,18-dihydroxylase activity. Comparisons of the crystal structure of Mt-CYP11B1 to those of Wt-CYP11B1 and CYP11B2 that were already reported show that the mutation reduced the innermost space putatively surrounding the C3 side of substrate 11-deoxycorticosterone (DOC) bound to Wt-CYP11B1, while the corresponding space in CYP11B2 is enlarged markedly and accessible to bulk water through a channel. Molecular dynamics simulations of their DOC-bound forms supported the above findings and revealed that the enlarged space of CYP11B2 had a hydrogen bonding network involving water molecules that position DOC. Thus, upon positioning 11 beta-hydroxysteroid for 18-hydrox-ylation in their substrate-binding sites, steric hindrance could occur more strongly in Mt-CYP11B1 than in Wt-CYP11B1 but less in CYP11B2. Our investigation employing Mt-CYP11B1 sheds light on the divergence in structure and function between CYP11B1 and CYP11B2 and suggests that CYP11B1 with spatially-restricted substrate-binding site serves as 11 beta-hydroxylase, while CYP11B2 with spatially-extended substrate-binding site successively processes additional 18-hydroxylations to produce aldosterone.
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关键词
Adrenal, Cytochrome P450, Molecular dynamics, Steroid hormone, X-ray crystallography
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