Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)

An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110 T , was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110 T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541 T (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491 T (99.1%), Streptomyces luteoverticillatus NBRC 3840 T (99.1%), Streptomyces coerulescens NBRC 12758 T (99.1%), and Streptomyces iranensis HM 35 T (99.0%). Strain CA3R110 T contained LL -diaminopimelic acid in cell peptidoglycan, MK-9(H 6 ), and MK-9(H 8 ) as major menaquinone, iso -C 16:0, iso -C 15:0, C 16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces . A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110 T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110 T represents a novel species of genus Streptomyces , for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110 T (= TBRC 11245 T = NBRC 114296 T ).