Y The Effect Of Contrast Enhanced Abdominopelvic Magnetic Resonance Imaging On Expression And Methylation Level Of Atm And Akt Genes

Objective: To evaluate the effect of contrast enhanced abdominopelvic magnetic resonance imaging (MRI), using a 3 Tesla scanner, on expression and methylation level of ATM and AKT genes in human peripheral blood lymphocytes.Materials and Methods: In this prospective in vivo study, blood samples were obtained from 20 volunteer patients with mean age of 43 +/- 8 years (range 32-68 years) before contrast enhanced MRI, 2 hours and 24 hours after contrast enhanced abdominopelvic 3 Tesla MRI. After separation of mononuclear cells from peripheral blood, using Ficoll-Hypaque, we analyzed gene expression changes of ATM and AKT genes 2 hours and 24 hours after MRI using quantitative reverse transcription polymerase chain reaction (qRT-PCR). We also evaluated methylation percentage of the above mentioned genes in before, 2 hours and 24 hours after MRI, using MethySYBR method.Results: Fold change analysis, in comparison with the baseline, respectively showed 1.1 +/- 0.7 and 0.8 +/- 0.5 mean of gene expressions in 2 and 24 hours after MRI for ATM, while the results were 1.4 +/- 0.6 and 1.4 +/- 1 for AKT (P>0.05). Methylation of the ATM gene promoter were 8.8 +/- 1.5%, 9 +/- 0.6% and 9 +/- 0.8% in before contrast enhanced MRI, 2 and 24 hours after contrast enhanced MRI, respectively (P>0.05). Methylation of AKT gene promoter in before contrast enhanced MRI, 2 hours and 24 hours after contrast enhanced MRI was 5.4 +/- 2.5, 5 +/- 3.2, 4.9 +/- 2.9 respectively (P>0.05).Conclusion: Contrast enhanced abdominopelvic MRI using 3 Tesla scanner apparently has no negative effect on the expression and promoter methylation level of ATM and AKT genes involved in the repair pathways of genome.