In vitro three-dimensional cell cultures for bone sarcomas

•3D cell cultures present similar drugs resistance features than in vivo tumours.•Unlike 2D cell cultures, 3D culture approaches mimic better the tumour microenvironment.•Collagen scaffolds result in a better osteosarcoma 3D proliferation than soft hydrogels.•Osteosarcoma 3D models constitute an appealing approach for bone mineralisation studies.•Combination of microfluidic/bioprinting technology with bone 3D cultures as ideal tools to study bone sarcomas.