Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo

Denisa Jansova,Daria Aleshkina,Anna Jindrova,Rajan Iyyappan,An Qin,Guoping Fan,Andrej Susor

Journal of Molecular Biology（2021）

引用 7|浏览7

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摘要

•Novel RNA-puro-PLA technique detects temporal and spatial translation of a single mRNA molecule in the subcellular context.•A significant correlation trend in expression was identified using multiple quantitative methods.•Translationally dormant and abundant Ccnb1 and Mos mRNAs clustering in the cytoplasm.

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关键词

mRNA,translation,imaging,localization,subcellular

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