Anther Culture of the Gametophytic Self-Incompatible Species Physalis ixocarpa Brot.
DOUBLED HAPLOID TECHNOLOGY, VOL. 2: Hot Topics, Apiaceae, Brassicaceae, Solanaceae(2021)
摘要
Here we present an optimized protocol for in vitro embryo formation and plant regeneration through anther culture of the Mexican husk tomato (Physalis ixocarpa Brot.). This protocol relies on the application of an anther thermal shock at a specific developmental stage prior to the in vitro culture, ensures embryo formation from anthers without callus formation, and allows spending less time to regenerate doubled haploid complete plants. This protocol has been used for different cultivars of Physalis ixocarpa (Chapingo, Rendidora, Puebla, Arandaz, Manzano, Tamazula, Salamanca, and Milpero), and also for two wild-type accessions, all of them cultivated in Mexico. Chapingo cultivar responded with the highest percentage of androgenesis on the embryo induction medium (EIM).
更多查看译文
关键词
Androgenesis, Anther culture, Doubled haploid, Physalis ixocarpa Brot
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要