Dentin non-collagen programmed preparation method

The invention provides a dentin non-collagen programmed preparation method. The method comprises the following steps: 1) collecting fresh non-dental caries tooth, cleaning the tooth and preserving thetooth in sodium azide with concentration being 15 mmol/L for several days, wherein sodium azide is taken as an antiseptic and a plurality of enzyme inhibitors; 2) flushing the tooth with running water for several hours and preserving the tooth at the temperature of -80 DEG C for standby; 3) unfreezing the tooth at room temperature, removing all soft tissues, cleaning the material; 4) using a dental drill or an operating knife to remove root cementum; 5) using a diamond bone saw or a low-speed cutting machine for cutting the tooth to small sheets; 6) removing pulp tissue and cells; 7) using abone knife or the bone saw to remove enamel; and 8) grinding the dentin sheet to fine powder. The method has the following beneficial results that 1) through a simple making technology, a lot of non collagen used for regenerating the subsequent related dentin induced tissues can be produced; and 2) a prepared dentin non-collagen mixture can simulate the induction action of the natural dentin in the pulp tissue regeneration.