The CDC crossmatch in the era of flow cytometric crossmatch and single antigen beads

The complement-dependent cytotoxicity crossmatch (CDC-XM), a technique that uses T and B lymphocytes to detect donor-specific antibodies (DSA) via activation of the classical pathway of complement, has been widely used for the detection of alloantibodies in transplant patients since it was introduced in the 1960s. In their seminal paper published in 1969, Patel and Terasaki demonstrated 24 out of 30 immediate graft failures in kidney transplants performed across a positive crossmatch, compared to eight out of 195 transplanted across a [...]