An Effective, Improved and Efficient Method of DNA Extraction from The New and Old Leaves of Mango (Mangifera indica L.)

Mango (Mangifera indica L.) is favoured for its delicious and juicy nature of the fruit and one of the important fruits of the world. It is commonly known as the “king of fruits”. The present experiment was designed to develop a simple method to obtain good quality as well as a higher yield of DNA (Deoxyribonucleic Acid) from the leaves of ten mango cultivars at two different stages (new flush and old leaf). This modified extraction method of CTAB (Cetyl Trimethylammonium Bromide) has the ability to isolate the required quality of DNA from mango old leaves, which contains higher amounts of polysaccharides and polyphenols. This method of DNA extraction is useful when the young leaves are not available year-round. Extracted DNA samples of ten cultivars were used to PCR (Polymerase Chain Reaction) amplification by using SSR (Simple Sequence Repeat) primer Mng SSR-14 showed clearly visible DNA bands in new flush and old leaves.