Self-assembled small messenger RNA nanospheres for efficient therapeutic apoptin expression and synergistic Gene-Chemotherapy of breast cancer

Small self-assembled messenger RNA nanospheres (mRNA-NSs) are successfully prepared by rolling circle transcription on a constructed apoptin plasmid. They can improve the expression of therapeutic protein apoptin and exhibit excellent synergistic anti-tumor effect after loading Dox, providing new ideas for the gene treatment and chemotherapy of breast cancer.