Glial-derived neurotrophic factor in human airway smooth muscle

Airway smooth muscle (ASM) cells modulate the local airway milieu via production of inflammatory mediators and growth factors including classical neurotrophins, such as brain-derived neurotrophic factor (BDNF). The glial cell-derived neurotrophic factor (GDNF) family of ligands (GFLs) are nonclassical neurotrophins and their role in the airway is barely understood. The major GFLs, GDNF and Neurturin (NRTN) bind to GDNF family receptor (GFR) alpha 1 and alpha 2 respectively that pair with Ret receptor to accomplish signaling. In this study, we found GDNF is expressed in human lung and increased in adult asthma, while human ASM expresses GDNF and its receptors. Accordingly, we used human ASM cells to test the hypothesis that ASM expression and autocrine signaling by GFLs regulate [Ca2+](i). Serum-deprived ASM cells from non-asthmatics were exposed to 10 ng/ml GDNF or NRTN for 15 min (acute) or 24 h (chronic). In fura-2 loaded cells, acute GDNF or NRTN alone induced [Ca2+](i) responses, and further enhanced responses to 1 mu M ACh or 10 mu M histamine. Ret inhibitor (SPP86; 10 mu M) or specific GDNF chelator GFR alpha 1-Fc (1 mu g/ml) showed roles of these receptors in GDNF effects. In contrast, NRTN did not enhance [Ca2+](i) response to histamine. Furthermore, conditioned media of nonasthmatic and asthmatic ASM cells showed GDNF secretion. SPP86, Ret inhibitor and GFR alpha 1-Fc chelator markedly decreased [Ca2+](i) response compared with vehicle, highlighting autocrine effects of secreted GDNF. Chronic GDNF treatment increased histamine-induced myosin light chain phosphorylation. These novel data demonstrate GFLs particularly GDNF/GFR alpha 1 influence ASM [Ca2+](i) and raise the possibility that GFLs are potential targets of airway hyperresponsiveness.