Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes

Julianne R Brown, Denise M O'Sullivan,Divya Shah, Laura Atkinson,Rui P A Pereira,Alexandra S Whale,Eloise J Busby,Jim F Huggett,Kathryn Harris

Journal of Virological Methods（2021）

引用 17|浏览4

暂无评分

摘要

•Multiple N-gene RT-PCR targets and commercial mastermixes evaluated.•In silico analysis shows good homology to available SARS-CoV-2 sequences.•Performance varies between PCR targets and mastermixes.•Some assays perform well regardless of mastermix used.•Poorer performing assays can be improved with appropriate choice of mastermix.

查看译文

关键词

SARS-CoV-2 RT-PCR,Mastermix,N gene

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要