Primer composition for identification of human adenovirus type 55 and application thereof

user-607cde9d4c775e0497f57189(2015)

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摘要
The invention discloses a primer composition for identification of human adenovirus type 55 and application thereof. The primer composition provided by the invention includes a DNA fragment-I, II, a DNA fragment-II, a DNA fragment-III and a DNA fragment-IV, all the fragments are single-stranded DNA fragments and are shown as sequence 1, sequence 2, sequence 3 and sequence 4 in order. The primer composition can also include a single-stranded DNA fragment-V and/or a single-stranded DNA fragment-VI, and the fragments are shown as sequence 5 and sequence 6 in order. The primer composition provided by the invention is applicable to specific detection and quantitative analysis on the human adenovirus type 55. The primer composition provided by the invention has the advantages of: (1) amplification reaction just under constant temperature and no need for special equipment; (2) high specificity; (3) fast speed and high efficiency, completion of the amplification reaction in a short period of time; (4) high sensitivity; and (5) convenient and simple identification. The primer composition provided by the invention is especially suitable for rapid detection of viruses in clinical samples.
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关键词
Primer (molecular biology),DNA,Sequence (medicine),A-DNA,Composition (combinatorics),Biochemistry,Chemistry,Fast speed,Rapid detection,Specific detection
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