Rna Sequencing Data For Gamma Radiation Response In The Extremotolerant Tardigrade Ramazzottius Varieornatus

Tardigrades are microscopic animals of which terrestrial species are capable of tolerating extreme environments by entering a desiccated ametabolic state known as anhydrobiosis. Intriguingly, they survive high dosage gamma rays (>4,000 Gy), possibly through a mechanism known as cross-tolerance. We hypothesized that anhydrobiosis genes are also regulated during cross-tolerance, thus we submitted Ramazzottius varieornatus to 500 Gy Co-60 gamma-ray and conducted time-course low-input RNA-Seq. The gene expression was quantified with RSEM and differential expression was determined with DEseq2. Differentially expressed genes were submitted to gene ontology enrichment analysis with GO-Stat. The transcriptome dynamically shifted nine hours post-exposure. (C) 2021 The Author(s). Published by Elsevier Inc.