Enhanced characterization of the thyA system for mutational analysis in Escherichia coli: Defining mutationally “hot” regions of the gene

Daniel Mashiach,Erin Mae Bacasen,Sunjum Singh,Timothy Kao,Lekha Yaramada,Daniel Mishail,Summer Singh,Jeffrey H Miller

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis（2021）

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摘要

•The thyA system in E. coli now allows detection of 343 base substitution mutations.•Mutability of regions is seen by comparing mutations found to the density of sites.•There are “hot” mutagenic regions of the gene at intervals between “cold” regions.

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关键词

Mutation,Hotspots,thyA system,Catalog,Cisplatin

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