Generation Of A Heterozygous Gapdh-Luciferase Human Esc Line (Hvrde008-A-1) For In Vivo Monitoring Of Stem Cells And Their Differentiated Progeny

Human stem cell-derived beta (SC-beta) cells are a candidate for cell replacement therapy for type 1 diabetes. Whilst refinements to the differentiation protocol have resulted in the production of SC-beta cells that resemble adult beta cells, the unsolved challenge to protect transplanted SC-beta cells from the host immune system remains. To monitor the survival of SC-beta cells in vivo, we knocked-in the Firefly luciferase gene into the GAPDH locus of the HUES8 human embryonic stem cell (hESC) line, such that differentiated islet cells constitutively express luciferase.