Development of a specific anti-idiotypic nanobody for monitoring aflatoxin M1 in milk and dairy products

Chong Cai, Qi Zhang, Seyni Nidiaye,Honglin Yan,Wen Zhang,Xiaoqian Tang,Peiwu Li

Microchemical Journal（2021）

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摘要

•An immune phage-displayed library was built with mAb against AFM1 as immunogens.•A sensitivity and specificity nanobody (VHH C4) was firstly screened through phage display technology.•A toxin-free competitive ELISA based on VHH C4 was built for the detection of AFM1 in milk and dairy products.

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关键词

Nanobody,Aflatoxin M1,Phage-displayed,ELISA,Milk products

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