Atypical kinetics of cytochrome P450 2J2: Epoxidation of arachidonic acid and reversible inhibition by xenobiotic inhibitors

•CYP2J2 metabolism of arachidonic acid (AA) to epoxyeicosatrienoic acids display atypical substrate inhibition kinetics.•Simultaneous and stable binding of two AA to CYP2J2 increased distance of ω6 double bond to heme-iron.•Substrate inhibition persists even at high concentrations of xenobiotic inhibitors.•Atypical kinetics and substrate-dependency affect inhibitory constant (Ki) values against CYP2J2.•Physiological substrate should be used to characterize xenobiotic-endobiotic interactions.