Streptococcus shenyangsis sp. nov., a New Species Isolated from the Oropharynx of a Healthy Child from Shenyang China

A Gram-positive, catalase-negative, coccus-shaped, chain-forming organism isolated from the oropharynx of a healthy child in Shenyang, China, was subjected to phenotypic and molecular taxonomic analyses. 16S rRNA sequence analysis indicated that this bacterium represents a new member of the genus Streptococcus and is closely related to Streptococcus oralis subsp. dentisani DSM 27088. According to DNA-DNA hybridization analysis, strain D19 T was less than 70% similar to other strains with close genetic relationships. Fatty acid analysis, physiological, and biochemical tests showed that strain D19 T was different from the published Streptococcus species. The genome of strain D19 T is 2,023,003 bp long with a GC content of 39.9 mol%. It contains 1889 protein-coding genes and 50 RNA genes. These results show that Streptococcus shenyangsis sp. nov. strain D19 T is a new species.