Multiclass pesticide residue analysis in tobacco (Nicotiana tabacum) using high performance liquid chromatography-high resolution (Orbitrap) mass spectrometry: A simultaneous screening and quantitative method

•First comprehensive screening & quantitation study on pesticide residues in tobacco matrix.•Non-target screening of pesticide residues by HPLC-quadrupole-orbitrap mass spectrometry.•Variable data independent acquisition offered accurate mass based screening & quantitation.•Isomeric & isobaric compounds differentiated based on LC separation & unique product ions.•Screening Detection Limits & LOQs in line with the current Guidance Residue Levels.