The conformational structural change of β-lactoglobulin via acrolein treatment reduced the allergenicity

•Acrolein induced structural changes through the cross-linking of BLG.•The IgE binding capacity of BLG was reduced upon acrolein treatment.•Stimulation with acrolein-treated BLG decreased RBL-2H3 cells degranulation rates.•BLG-specific IgE/IgG1, histamine and mMCP-1 levels were reduced in mice model.•Structural changes resulted in reduction of BLG allergenicity by lipid peroxidation.