Phenotypic Characterization Of Sulfolobus Islandicus Strains Lacking The B-Family Dna Polymerases Polb2 And Polb3 Individually And In Combination
FRONTIERS IN MICROBIOLOGY(2021)
摘要
Across the three domains of life, B-family DNA polymerases play a variety of roles in both DNA repair and DNA replication processes. We examine the phenotypic consequences of loss of the putative repair polymerases PolB2 and/or PolB3 in the crenarchaeon Sulfolobus islandicus. We detect a modest growth advantage when cells lacking the polymerase are grown in unperturbed conditions. Further, we observe a striking insensitivity of the mutant lines to acute treatment with the oxidizing agent, hydrogen peroxide. In addition, cells lacking PolB3 show enhanced sensitivity to the DNA damaging agent 4-NQO. Our data therefore suggest that these non-essential DNA polymerases may influence DNA repair pathway choice in these hyperthermophilic aerobes.
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关键词
Sulfolobus, archaea, DNA replication, DNA damage, polymerase
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