Absence Of Estrogen Receptors Delays Myoregeneration And Leads To Intermuscular Adipogenesis In A Low Estrogen Status: Morphological Comparisons In Estrogen Receptor Alpha And Beta Knock Out Mice

This study aimed to investigate the function of estrogen receptors (ERs) in myoregeneration and intermuscular adipogenesis. Ovariectomized (OVX) ER alpha knockout (KO) mice and ER beta KO mice were used to assess the effect of estrogen on the myoregenerative process. Tibialis anterior muscle was collected on days 7, 10, and 14 after cardiotoxin injection to assess myotube morphology and adipogenesis area. Regenerated myotubes from OVX-ER beta KO mice were consistently smaller in diameter than those from OVX-ER alpha KO and OVX-wild-type mice, whereas the adipogenesis area of OVX-ER beta KO mice was consistently greater than that of the other types. Therefore, ER beta may be an influential factor in promoting myoregeneration and adipogenesis inhibition compared to ER alpha.