Assessment Of Mixed Plasmodium Falciparum Sera5 Infection In Endemic Burkitt Lymphoma: A Case-Control Study In Malawi

Simple Summary: Plasmodium falciparum(Pf) infection is a risk factor for endemic Burkitt lymphoma (eBL), the commonest childhood cancer in Africa, but the biomarkers of Pf infection that predict this risk are unknown. There is some evidence that the genetic complexity of Pf infection may be a risk factor. In 200 children with versus 140 without eBL in Malawi, this study compared variants of the malaria parasite, focusing on Pfsera5, a gene that codes for malaria protein that an infected person's antibodies target to suppress the parasite. Multiple Pfsera5 variants, which arise when the parasite is not suppressed, were found in 41.7% of eBL children versus 24.3% of other local children, meaning that eBL risk was increased 2.4-fold with multiple Pfsera5 variants. No specific type of variant was related to eBL risk. Research to quantify malaria parasite variants and to clarify the host immune response needed to control variant infections may yield a test to predict eBL risk.Background: Endemic Burkitt lymphoma (eBL) is the most common childhood cancer in Africa and is linked to Plasmodium falciparum (Pf) malaria infection, one of the most common and deadly childhood infections in Africa; however, the role of Pf genetic diversity is unclear. A potential role of Pf genetic diversity in eBL has been suggested by a correlation of age-specific patterns of eBL with the complexity of Pf infection in Ghana, Uganda, and Tanzania, as well as a finding of significantly higher Pf genetic diversity, based on a sensitive molecular barcode assay, in eBL cases than matched controls in Malawi. We examined this hypothesis by measuring diversity in Pf-serine repeat antigen-5 (Pfsera5), an antigenic target of blood-stage immunity to malaria, among 200 eBL cases and 140 controls, all Pf polymerase chain reaction (PCR)-positive, in Malawi. Methods: We performed Pfsera5 PCR and sequencing (similar to 3.3 kb over exons II-IV) to determine single or mixed PfSERA5 infection status. The patterns of Pfsera5 PCR positivity, mixed infection, sequence variants, and haplotypes among eBL cases, controls, and combined/pooled were analyzed using frequency tables. The association of mixed Pfsera5 infection with eBL was evaluated using logistic regression, controlling for age, sex, and previously measured Pf genetic diversity. Results: Pfsera5 PCR was positive in 108 eBL cases and 70 controls. Mixed PfSERA5 infection was detected in 41.7% of eBL cases versus 24.3% of controls; the odds ratio (OR) was 2.18, and the 95% confidence interval (CI) was 1.12-4.26, which remained significant in adjusted results (adjusted odds ratio [aOR] of 2.40, 95% CI of 1.11-5.17). A total of 29 nucleotide variations and 96 haplotypes were identified, but these were unrelated to eBL. Conclusions: Our results increase the evidence supporting the hypothesis that infection with mixed Pf infection is increased with eBL and suggest that measuring Pf genetic diversity may provide new insights into the role of Pf infection in eBL.