Systematic Study Of Sybr Green Chromophore Reveals Major Improvement With One Heteroatom Difference
JOURNAL OF MATERIALS CHEMISTRY B(2021)
摘要
Five nucleic acid binding cyanine dyes were synthesized and their photophysical properties were evaluated. Changing a single heteroatom in the chromophore causes major differences both in brightness and photostability between the dyes. With such alteration, the brightness of the chromophore increased two-fold compared to the one found in SYBR Green I.
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