Pontixanthobacter Rizhaonensis Sp. Nov., A Marine Bacterium Isolated From Surface Seawater Of The Yellow Sea, And The Proposal Of Pseudopontixanthobacter Gen. Nov., Pseudopontixanthobacter Confluentis Comb. Nov. And Pseudopontixanthobacter Sediminis Comb. Nov. (Vol 71, 004780, 2021)

A bacterial strain designated RZ02(T) was isolated from surface seawater collected from the Yellow Sea in PR China and characterized by polyphasic taxonomy. Cells of strain RZ02(T) were Gram-stain-negative, aerobic, non-motile, catalase-and oxidase-positive rods forming ochre-pigmented colonies. Growth occurred at 7-36 degrees C (optimum, 30 degrees C), at pH 6.0-9.0 (optimum, pH 7.0) and with 1-5% (optimum, 2%) NaCl. The major cellular fatty acids (>10%) of strain RZ02(T) were summed feature 8 (C-18:1 omega 6c and/ or C-18:1 omega 7c), summed feature 3 ( C-16:1 omega 7c and/ or C-16:1 omega 6c) and C-16:0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and sphingoglycolipid. The genome size of strain RZ02(T) was 2.79 Mbp with a G+C content of 55.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ02(T) was mostly related to Pontixanthobacter luteolus SW-109(T) and Pontixanthobacter aestiaquae HDW-31(T) (97.3 and 97.1% sequence similarity, respectively), and formed a phyletic lineage with members of the genus Pontixanthobacter. The phylogenetic analysis based on the up-to-date bacterial core gene sequences confirmed that strain RZ02(T) clustered within the genus Pontixanthobacter. The average nucleotide identity and in silico DNA-DNA hybridization values between strain RZ02(T) and P. luteolus SW-109(T) and P. aestiaquae HDW-31(T) were 72.8 and 72.9 % and 18.7 and 18.5%, respectively. Based on these evidences, strain RZ02(T) is proposed to represent a novel species of the genus Pontixanthobacter under the name Pontixanthobacter rizhaonensis sp. nov. The type strain is RZ02(T) (=KCTC 62828(T)=MCCC 1K04521(T)). In addition, based on the results of whole genome analyses, proposals of Pseudopontixanthobacter gen. nov., Pseudopontixanthobacter confluentis comb. nov. and Pseudopontixanthobacter sediminis comb. nov. are also included.