Recombination Lactococcus Lactis Expressing Helicobacter Pylori Neutrophil-Activating Protein A Attenuates Food Allergy Symptoms In Mice

Background: Food allergy has been a significant public health issue with growing severity, prevalence and limited treatments. The neutrophil-activating protein A subunit (NapA) of Helicobacter pylon has been shown to have therapeutic potential in allergic diseases. Methods: The NapA expression efficiency of recombinant Lactococcus lactis (L.lactis) were determined. The effects of recombinant bacterium on food allergy in Balb/c mice were also investigated. Results: NapA were delivered and expressed efficiently via L. lactis. The engineered bacterium ameliorated food allergy symptoms (acute diarrhea and intestinal inflammation) and decreased serum histamine levels. In addition, the secretion of OVA-specific IgG2a, IFN-gamma was promoted and the level of IL-4, OVA-specific IgE was restrained. Conclusions: The recombinant strain may attenuate food allergy in mice through immune regulatory effect, which may be a promising approach for preventing or treating food allergy.