Monitoring single tumor interactions with fibroblasts in autophagy activation

In tumor microenvironment, tumors interact with stromal cells such as immune cells, mesenchymal stem cells and fibroblasts etc. Specifically, communication with fibroblasts is related to tumor survival, proliferation and metastasis. We developed a high throughput screening platform based on a single tumor cell-to-fibroblasts interaction and monitored kinetics of GFP-LC3 positive fibroblasts by single tumor. Our research can have a potential to monitor single tumor which can activate autophagy in fibroblasts and help to study the heterogeneity of tumor and mechanism between tumor and fibroblast in autophagy.