Aquabacterium terrae sp. nov., isolated from soil

A yellow-colored bacterial strain, designated S2 T was isolated from soil in South Korea. Cells of strain S2 T were strictly aerobic, Gram-stain-negative, motile with single polar flagellum, rod-shaped, oxidase and catalase-negative. Growth occurs at 10–37 °C (optimum, 28 °C), pH 5.0–9.0 (optimum, pH 6.5–7.0) and 0–3% NaCl (w/v). Strain S2 T consisted of summed feature 3 (iso-C 15:0 2-OH and/or C 16:1 ω 7 c ), C 16:0 and summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ) as major fatty acids. The sole respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine and an unidentified lipid. The 16S rRNA gene sequence analysis showed that strain S2 T is phylogenetically closest to Aquabacterium pictum W35 T (98.4% sequence similarity). The genome of strain S2 T was 8,039,486 bp with 56 scaffolds. The genome consisted of 10 putative biosynthetic gene clusters that are responsible for various secondary metabolites. Genomic DNA G + C content of strain S2 T was 69.4%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain S2 T and phylogenetically related taxa were ≤ 77.9 and ≤ 21.4%, and respectively. The results of genotypic and phenotypic data showed that strain S2 T could be distinguished from its phylogenetically related species and represents a novel species in the genus Aquabacterium , for which the name Aquabacterium terrae sp. nov. is proposed. The type strain is S2 T (= KCTC 72741 T = NBRC 114609 T ).