IMMOBILIZATION OF AMYLASE PRODUCING BACTERIA (BACILLUS SUBTILIS) FROM SOIL OF WESTERN HIMALAYAN REGION SOLAN, HIMACHAL PRADESH, INDIA

Objective: The paper aimed to immobilize amylase producing bacterial strain on a suitable matrix and characterization of its physicochemical properties so that much amount of amylase could be produced to be applied in different industries. Methods: Bacterial colonies were sub-cultured from samples collected from soil in freshly prepared dishes containing starch agar by dot method using sterile inoculating needles from which five different bacteria belonged to genus Bacillus were isolated and assigned as A1, A2, A3, A4, and A5. Results: It was found that A1 displayed the highest enzyme activity of 17.89 IU/ml with enzyme assay of 0.83 mg/ml and the bacterium was identified to be Bacillus subtilis. A5 displayed 10.13 IU/ml with protein contents of 0.11 mg/ml indicated that A1 possess the highest enzyme activities which were categorized under Bacillus and protein contents and A5 showed less amount of enzyme activities and protein contents as compared to other. Conclusion: The bacteria which were produced much amount of enzyme activities identified as Bacillus subtilis and recommended and have been recommended to be cultured for the production of amylase enzyme.