A Simple Strategy For Constructing Pet Fluorescent Probe And Its Application In Hypochlorite Detection

It is great meaningful to develop a fast and efficient method for detecting hypochlorite (ClO-) owing to its importance in the immune system. In this work, we proposed a strategy to construct fluorescent probes for ClO- based on photoinduced electron transfer (PET) mechanism. According to the strategy, we developed four fluorescent probes named TPA-NO2, TPA-2NO(2), TPB-NO2 and TPB-2NO(2), and studied their detecting performances for hypochlorite. Among them, TPB-NO2 displayed the most obvious fluorescence changes towards ClO- with a rapid response (<90 s). The detection limit was calculated to be 0.36 lM. Moreover, probe TPB-NO2 was successfully used to detect ClO- in living cells and zebrafish. These results demonstrated the feasibility of our strategy and provided a guidance for developing more excellent probes in the future. (C) 2021 Elsevier B.V. All rights reserved.