Application Of Deep Eutectic Solvents Combined With Vortex Assisted Dispersive Liquid-Liquid Microextraction For Five Organophosphorus Pesticides In Juice And Green Tea Beverage

In this study, a new microextraction method based on hydrophobic deep eutectic solvents was developed for the extraction and preconcentration of organophosphorus pesticides from beverage samples. The hydrophobic deep eutectic solvents were synthesized from choline chloride and 4-chlorophenol. Main experimental parameters of the microextraction method were investigated to improve the extraction efficiency. The proposed method achieved a satisfactory linear range between 50 and 2,000 mu g L-1 with coefficient of determination greater than 0.9939. The extraction recoveries and enrichment factor of five organophosphorus pesticides at three added levels ranged from 71.68 to 113.18% and 71.43-111.11 were obtained with the acceptable relative standard deviation ranged from 1.37 to 11.92%. Limits of detection and quantification were found to be 0.05-0.3 mu g L-1 and 0.17-1 mu g L-1, respectively. Finally, the methods were successfully applied for the determination of five organophosphorus pesticide in orange juice and green tea.