New compounds from the stems of Fissistigma oldhamii var. longistipitatum and their cytotoxic activities

Two new aporphine alkaloids, (R)-1,2-methylenedioxy-3,9-dimethoxy-11-hydroxy-N- carbamoyl-noraporphine (1) and 3,10,11-trimethoxy-1,2-methylenedioxy-7-oxoaporphine(2), and one new dihydrochalcone, 4′,5′-dimethoxy-2′-hydroxy-3′,6′-quinodihydrochalcone (3), along with five known alkaloids were isolated from the ethanol extracts of the stems of Fissistigma oldhamii var. longistipitatum. The compounds were obtained by various classical column chromatographic methods, and the structure elucidation was completed primarily on the basis of spectroscopic analysis, such as UV, NMR and HR-ESI-MS. The isolated compounds were subjected to evaluate cytotoxic activities in vitro, compound 1 had activity against HL-60 and HELA (IC50 value of 8.4 μM and 5.2 μM, respectively), compound 2 against MCF-7 (IC50 value of 3.7 μM), compound 3 against HEPG2 (IC50 value of 10.8 μM), respectively.