Metabolites Analysis Of Single Cell By Mass Spectrometry And Application In Single Neuron Metabolites Analysis

Single-cell assays has emerged as a cutting-edge technique during the past decade. Despite several issues (including pL volume, extremely complicated intracellular fluid and maintaining cell viability during sampling) still need to be addressed, single-cell mass spectrometry for metabolites has achieved remarkable results recently. Various mass spectrometry-based approaches have enabled identification of vast cytoplasmic chemical constituents at single cell level, which greatly facilitate the single neuron analysis at different physiological conditions. In this review, we have concluded recent single-cell mass spectrometry investigations toward single neuron analysis, including single ovum/zygote, single cultured cell, single freshly isolated cell and single cell/neuron on tissue.