Determination Of 22 Kinds Of Persistent Organic Pollutants In Serum Of Pregnant Women By Matrix Solid Phase Dispersion Procedure And Gas Chromatography Coupled To Triple Quadrupole Mass Spectrometry

A method was developed for the simultaneous determination of 22 kinds of persistent organic pollutants (POPs) (Aldrin, Dieldrin, Mirex, HCHs, DDTs and PCBs) in serum of pregnant women by matrix solid phase dispersion procedure and gas chromatography coupled to triple quadrupole mass spectrometry (MSPD-GC-QQQ-MS/MS). The serum samples were blended with C-18 adsorbent and eluted by n-hexane. Agilent DB-35MS column (30 mx0.25 mmx0.25 mu m) was used to separate 22 kinds of POPs with high sensitivity and satisfactory resolution. The identification and quantification were achieved by using electron ionization in positive ion mode (EI+) with multiple reaction monitoring (MRM). The results showed that matrix. matched calibration curves with good correlation coefficients (R-2 > 0.99) were obtained in the concentration range of 1.0 - 1000.0 ng/mL. The recoveries of the spiked samples at 2.0 ng/mL and 20.0 ng/mL were in the range of 75% to 115% with the RSD lower than 13%. The limits of quantification (LOQ) for 22 kinds of POPs were. 0.15 ng/mL. Liquid. liquid extraction (LLE) and solid phase extraction (SPE) were used to compare with the MSPD method by spiked serum and the efficiency of the methods mentioned above was evaluated by determining the concentration of POPs (ng/mg lipid). Median value obtained by MSPD method was the closest to actual concentration, and the dispersion of all data was minimal. The method was successfully applied to the simultaneous determination of POPs for quantification and confirmation in human serum.