Mesenchymal Stem Cells Affect Polarization Of Macrophage Phenotype Damaged Due To Chronic Inflammation Via The Cox-2 Pathway

Objective To study the effect of macrophage phenotype and mesenchymal stem cells (MSC) on polarization of macrophage phenotype damaged due to combined high glucose and inflammation.Methods Primarily cultured peritoneal macrophages from rats were divided into normal group,damaged group (33 mmol/L glucose and 1 μg/ml lipopolysaccharide),treatment group (cocultured high glucose + Inflammation-damaged LPS + MSC transwell),and inhibitor group (MSC+COX-2 inhibitor).Morphology of macrophages was observed under optical microscope,M1 and M2 macrophages were detected by flow cytometry,and serum levels of IL-6,IL-10 and PGE2 were measured by ELISA.Results The ratio of M1 macrophages was significantly lower while that of M2 macrophages was significantly higher in treatment group than in damaged group (8.91％±0.71％ vs 16.19％±0.78％,P＜0.05;51.09％±4.89％ vs 28.10％±12.47％,P＜0.05).The serum level of IL-6 was significantly lower while that of IL-10 was significantly higher in treatment grup than in damaged group (P＜0.05).The ratio of M1 macrophages was significantly higher in inhibitor group than in treatment group (19.94％ ± 2.07％ vs 8.91％ ± 0.71％,P＜0.05).The serum level of IL-6 was significantly higher while that of IL-10 was significantly lower in inhibitor group than in treatment group (P＜0.05).Conclusion MSC can promote the M1 macrophaes damaged due to combined high glucose and inflammation to the polarization of M2 macrophage phenotype and repair the damaged macrophages through the COX-2 pathway.