Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells

Venugopal Bhaskara, Maria Trinidad Leal, Jacqueline Seigner, Theresa Friedrich,Emanuel Kreidl,Elisabeth Gadermaier,Manfred Tesarz, Azra Rogalli, Laura Stangl,Jacqueline Wallwitz,Katharina Hammel,Mario Rothbauer,Herwig Moll, Peter Ertl,Rainer Hahn,Gottfried Himmler,Anton Bauer,Emilio Casanova

Journal of Biotechnology（2021）

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摘要

•Establishment of a fast and reliable expression system in CHO-K1 cells that allows to develop cell lines in six weeks expressing dimeric IgAs and glyco-engineered human secretory component, hSC.•Establishment of a protocol to purify dimeric IgAs and the hSC.•Establishment of a protocol to reconstitute SIgA.•Validation of the functionality of two dimeric and secretory IgAs against toxins TcdA and TcdB produced with our system.

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关键词

Secretory IgA,Dimeric IgA,BAC-based expression system,Recombinant protein production,CHO-K1 cells,Flow cytometry sorting

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