Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development.

Matthias Jung,Jovita Schiller, Carla Hartmann,Jenny Pfeifer,Dan Rujescu

IN VITRO MODELS FOR STEM CELL THERAPY: Methods and Protocols（2021）

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摘要

We describe the protocol for the efficient in vitro differentiation of human neural stem cells (NSCs) from human-induced pluripotent stem cells (iPS cells). NSCs differentiate via neural epithelial progenitors enabling the analysis of early neuronal development. They represent neural progenitor cells, which are capable of differentiating into neurons and glia.

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关键词

Human neural stem cells, Induced pluripotent stem cells, Neural epithelial progenitors, Early neuronal development, Embryoid bodies, Neurospheres

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