Universal primer for detecting aspergillus flavus and aspergillus fumigatus and real-time fluorescence tHDA (Thermophilic Helicase Dependent Amplification) kit

The invention discloses a universal primer for detecting aspergillus flavus and aspergillus fumigatus and a real-time fluorescence tHDA (Thermophilic Helicase Dependent Amplification) kit. The universal primer can be used for simultaneously detecting the aspergillus fumigatus and the aspergillus flavus in an amplification manner and is simultaneously combined with a real-time fluorescence tHDA technology. Thus, compared with other common PCR (Polymerase Chain Reactions), according to the universal primer, the amplification can be carried out at a constant temperature, so that the processes of denaturation, annealing, extending and the like are omitted, i.e., the helicase amplification can be carried out by directly simulating a biology environment, which is favorable for simplification of equipment and popularization of the universal primer in grass roots. The detection limit of the real-time fluorescence tHDA kit can reach 104copies/ml, so that the real-time fluorescence tHDA kit is suitable for clinical applications. The real-time fluorescence tHDA kit can be used for detecting two types of aspergillus infections which are the most common in clinical, so that a large quantity of precious rescue time in clinical can be saved and the kit is low in cost and convenient to operate. Thus, the real-time fluorescence tHDA kit has a wide application prospect.