LN-229 cell line with low expression of human phosphatase and tensin homolog (PTEN) protein and construction method for LN-229 cell line

The invention relates to a construction method for a LN-229 cell line with low expression of phosphatase and tensin homolog (PTEN). The stable LN-229 cell line with low expression of PTEN is constructed by utilizing a lentivirus system; shPTEN and lentivirus packaging plasmids are transfected into HEK293T cells; a virus supernatant is collected to infect LN-229 cells; the transfected LN-229 cellsare screened with puromycin to obtain the stable LN-229 cell line with low expression of PTEN; and finally, tested by a Western blot technique, the expression of PTEN of cell strains is decreased by more than 80%, and thus, the cell line with low expression of PTEN is successfully constructed. The cell line can serve as an ideal cell line for researching the molecular action mechanism and the participating metabolic regulation effect of PTEN in glioma.