Age-Dependent Messenger Rna Expression Of Toll-Like Receptor 4 And Intercellular Adhesion Molecule-1 In Peripheral Blood Mononuclear Cells

Background Inflammation plays an important role in the ageing process in which monocytes/macrophages are important players. Intercellular adhesion molecule-1 (ICAM-1), tumour necrosis factor-alpha (TNF-alpha) and Toll-like receptor 4 (TLR4) are well-known inflammatory markers. This study aimed to investigate the relationship between age and the expression and correlation of ICAM-1, TNF-alpha and TLR4 mRNA in peripheral blood mononuclear cells (PBMCs).Methods A total of 239 participants were recruited in a medical centre in Taiwan. The mRNA isolated from the PBMCs was used to determine the levels of ICAM-1, TNF-alpha and TLR4 mRNAs with real-time polymerase chain reaction (PCR). The propensity-matched analysis was also applied for subgroup analysis.Results When compared 189 older adults (>= 65 years) to 50 younger adults (<65 years), the ICAM-1, TNF-alpha and TLR4 mRNA levels in PBMCs were significantly higher in older adults (2.00 +/- 0.72 vs 0.87 +/- 0.34 for ICAM-1, 2.32 +/- 0.69 vs 1.15 +/- 0.44 for TNF-alpha and 1.56 +/- 0.47 vs 1.05 +/- 0.51 for TLR4, and all P < .0001). Also, both age and TLR4 were independent factors affecting mononuclear cell ICAM-1 in the multiple linear regression analysis (P < .0001).Conclusion The mRNA levels of ICAM-1 and TLR4 in PBMCs are higher in older adults than those in younger adults. TLR4 is an independent factor affecting ICAM-1 expression in PBMCs, especially in older adults. This may suggest that ICAM-1 and TLR4 in PBMCs are potential biomarkers and their relationship may shed some light on the ageing process.