Synthesis, In-Vitro Evaluation, Molecular Docking, And Kinetic Studies Of Pyridazine-Triazole Hybrid System As Novel Alpha-Glucosidase Inhibitors

In this study, we reported the discovery of pyridazine based 1,2,3-triazole derivatives as inhibitors of ?-glucosidase. All target compounds exhibited significant inhibitory activities against yeast and rat ?-glucosidase enzymes compared to positive control, acarbose. The most potent compound 6j, ethyl 3-(2-(1-(4-nitrobenzyl)-1H1,2,3-triazol-4-yl)ethyl)-5,6-diphenylpyridazine-4-carboxylate exhibited IC50 values of 58, and 73 ?M. Docking studies indicated the responsibility of hydrophobic and hydrogen bonding interactions in the ligand-enzyme complex stability. The in-vitro safety against the normal cell line was observed by toxicity evaluation of the selected compounds.