Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales.

Tomas Fessl,Joel A Crossley,Daniel Watkins,Marek Scholz,Matthew A Watson,Tara Sabir,Sheena E Radford,Ian Collinson,Roman Tuma

Methods in molecular biology (Clifton, N.J.)（2020）

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摘要

Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule Förster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10-10 s).

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关键词

FRET,Lipid,Liposome,Protein export,SecA,SecY,Translocation

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