Conditional Gene Silencing Via A Crispr System In Cerebellar Purkinje Cells
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS(2021)
摘要
Specific gene knockdown in cerebellar Purkinje cells is a major challenge because Purkinje cells only make up a small fraction of the cerebellum and off-target effects of shRNA occur.Little is known about the application of CRISPR-Cas13 and the resulting protein expression levels in Purkinje cells, a type of developing postmitotic neuron.We explored the possibility of a Cas13-mediated conditional knockdown system for cerebellar Purkinje cells. This system can achieve a suppression of the target proteins restricted to Purkinje cells in mixed cerebellar cultures.
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关键词
Purkinje cells,gene silencing
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