Microrna-199a-3p Inhibits Ovarian Cancer Cell Viability By Targeting The Oncogene Yap1

MicroRNA-199a-3p (miR-199a-3p) is aberrantly expressed in various types of cancer where it exhibits a tumor suppressive role. However, the biological role of miR-199a-3p in ovarian cancer (OC) remains unclear. The present study aimed to investigate whether miR-199a-3p was a tumor suppressor in OC and to identify the possible mechanisms. It was found that miR-199a-3p expression was significantly downregulated in the tumor tissues and blood samples of patients with OC, as well as in three OC cell lines. In addition, its low expression was closely associated with International Federation of Gynecology and Obstetrics disease stage, histological grade and lymph node metastasis. It was demonstrated that overexpression of miR-199a-3p inhibited the viability and promoted apoptosis of OV90 and SKOV-3 cells. In addition, Yes-associated protein 1 (YAP1), a well-known oncogene, was identified as a direct target of miR-199a-3p in OC cells. Additionally, it was observed that YAP1 was significantly increased and inversely correlated with miR-199a-3p expression in OC tissues. Notably, YAP1 overexpression abrogated the tumor suppressive effects of miR-199a-3p in vitro. Collectively, the present results indicated that miR-199a-3p suppressed viability in OC cells, at least partly via inhibiting the YAP1 oncogene, suggesting that miR-199a-3p may act as a biomarker and therapeutic target for patients with OC.