Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.

We have previously adapted a select number of cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in S2 cells.