Development Of A Monoclonal Antibody-Based Immunochromatographic Strip For The Rapid Detection Of Tigecycline In Human Serum

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and was found to have a 50%-inhibitory concentration (IC50) of 2.303 ng mL(-1) and a limit of detection (LOD) of 0.215 ng mL(-1) for TGC. This strip assay had a visual limit of detection (vLOD) of 50 ng mL(-1) and a cut-off value of 1000 ng mL(-1) for TGC in human serum, when assessed by eye. With the aid of a strip scan reader, a quantitative determination of TGC was obtained with a calculated limit of detection (cLOD) of 15.03 ng mL(-1). Analysis of TGC in human serum indicated that the results of our strip assay compared well with results obtained using ic-ELISA and LC-MS/MS. Therefore, this strip assay represents a sensitive and reliable method for the on-site detection of TGC in serum samples.