Effects of post-pyrolysis treated biochars on methane production, ruminal fermentation, and rumen microbiota of a silage-based diet in an artificial rumen system (RUSITEC)

•Biochar did not affect rumen fermentation and nutrient disappearance.•Biochar had no effect on total gas and methane production.•Biochar did not affect protozoa populations and microbial protein synthesis.•Biochar had no effect on rumen microbiota diversity and richness.