Discovery of the bioactive peptides secreted by Bifidobacterium using integrated MCX coupled with LC–MS and feature-based molecular networking

Food Chemistry(2021)

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摘要
•An integrated approach based on MCX-SPE coupled with LC–MS and FBMN was developed.•25 novel bioactive peptides were quickly characterized from Bifidobacterium.•Gypenosides-treated Bifidobacterium released 4 unique peptides.•A peptide HLPLPF was synthesized and showed ACE inhibitory effect.
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关键词
Bioactive peptides,Bifidobacterium,MCX coupled with LC–MS,Feature-based molecular networking
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