Rice Haploid Inducer Development by Genome Editing.

Juntao Liu,Dawei Liang, Li Yao,Ya Zhang,Chunxia Liu,Yubo Liu,Yanli Wang,Hongju Zhou,Timothy Kelliher,Xingping Zhang,Anindya Bandyopadhyay

RICE GENOME ENGINEERING AND GENE EDITING: Methods and Protocols（2021）

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摘要

The current method to induce haploids in rice is anther culture, which is time-consuming and labor intensive and only works for some varieties. Here we describe a seed-based haploid induction system created by CRISPR/Cas9 technology. By editing OsMATL, we generate rice haploid inducer lines with a 2-5% haploid induction rate in different germplasms.

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关键词

CRISPR/Cas9,Crossing,Genome editing,Haploid inducer,OsMATL,Pollination,Rice

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